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B026 |
FLUID SELENITE CYSTINE MEDIUM |
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Formula |
B026 |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Lactose Disodium phosphate Sodium hydrogen selenite L-Cystine |
5.00 4.00 10.00 4.00 0.01 |
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Final pH (at 25°C) : 7.0 + 0.2 |
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Directions |
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Suspend 23 gms. of B026 or in 1000 ml. distilled water. Warm to dissolve the medium completely. Distribute in sterile test tubes. Sterilize in a boiling water bath or free flowing steam for 10 minutes. DO NOT AUTOCLAVE. Excessive heating is detrimental. Discard the prepared medium if large amount of selenite is reduced indicated by red precipitate at the bottom of tube/bottle. |
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