B015 

BRILLIANT GREEN BILE BROTH 2%

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Lactose 

Oxgall 

Brilliant green 

10.00

10.00

20.00

0.0133

Final pH (at 25°C) : 7.2 + 0.2

Directions 

Suspend 40 gms. in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Distribute in fermentation tubes containing inverted Durham’s tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Do not autoclave double strength broth.

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