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B011 |
ALTERNATIVE THIOGLYCOLLATE MEDIUM |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Yeast extract Dextrose Sodium chloride L-Cystine Sodium thioglycollate |
15.00 5.00 5.50 2.50 0.50 0.50 |
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Final pH (at 25°C) : 7.1 + 0.2 |
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Directions |
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Suspend 29 gms. in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Distribute into tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 minutes. |
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