B004 

YEAST NITROGEN BASE W/O AMINO ACIDS AND 

AMMONIUM SULPHATE

Formula

Ingredients 

gms/lit.

Biotin

Calcium pantothenate

Folic acid

Inositol

Niacin

p-amino benzoic acid (PABA)

Pyridoxine hydrochloride

Riboflavin (vitamin B2)

Thiamine hydrochloride

Boric acid

Copper sulphate

Potassium iodide

Ferric chloride

Manganese sulphate

Sodium molybdate

Zinc sulphate

Monopotassium phosphate

Magnesium sulphate

Sodium chloride

Calcium chloride

0.000002

0.0004

0.000002

0.002

0.0004

0.0002

0.0004

0.0002

0.0004

0.0005

0.00004

0.0001

0.0002

0.0004

0.0002

0.0004

1.00

0.50

0.10

0.10

Final pH (at 25°C) : 4.5 + 0.2

Directions 

1. For Carbon Assimilation tests, prepare the broth base in 10X concentration. Dissolve 1.7 gms. in 100 ml. distilled water. Add 5 gms. ammonium sulphate, 10 mg L-Histidine,20 mg DL-methionine and 20 mg DL-tryptophan. Carbon compounds for assimilation test are added in 10X concentration singly or in combination as required.

2. For Nitrogen Assimilation tests, prepare the medium in 10X concentration. Dissolve 1.7 gms. in 100ml. distilled water. Add 1,0 gms. dextrose, 1 mg Lhistidine, 2 mg DL-methionine and 2 mg  DL-tryptophan. Add nitrogen compounds for assimilation test in 10X concentration singly or in combination as required. Wickerham employed the following nitrogen sources : ammonbium sulphate 1 gm. potassium nitrate 0.78 gms., urea 0.46 gms. aspargine 1 gm. peptone (gelatin) 1.32 gms.

For A and B, filter sterilize the 10X strength solution, Refrigerate and use as needed.Prepare final medium by aseptically pipetting 0.5 ml. of the 10X sterile medium into 4.5 ml. sterile distilled water. Mix Well

Print This Page

Close Window