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B1009 |
DICHLORAN GLYCEROL MEDIUM BASE |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Dextrose Monopotassium phosphate Magnesium sulphate Chloramphenicol Dichloran Agar |
5.00 10.00 1.00 0.50 0.10 0.002 15.00 |
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Final pH (at 25°C) : 5.6 + 0.2 |
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Directions |
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Suspend 15.8 gms. in 500 ml. distilled water. Heat to boiling to dissolve the medium completely. Add 110 gms. of glycerol (analytical Reagent Grade). Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. |
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