BW1044 

LYSINE DECARBOXYLASE BROTH without PEPTONE

Formula 

Ingredients 

gms/lit.

Yeast extract 

Dextrose 

L-lysine monohydrochloride

Bromo cresol purple 

3.00

1.00

5.00

0.015

Final pH (at 25°C) : 6.8 + 0.2

Directions 

Suspend 9 gms. in 1000ml. distilled water. Boil to dissolve the medium completely. Dispense 5 ml amount into screw capped test tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool the tubed medium in upright position and overlay with 2-3 ml.of sterile mineral oil.

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