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BS022 |
FOLIC ACID ASSAY MEDIUM (S. FAECALIS) |
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Formula |
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Ingredients |
gms/lit. |
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Casein Acid Hydrolysate, vitamin free Dextrose Sodium Citrate L-Cystine DL-Tryptophan Adenine Sulphate Guanine Hydrochloride Uracil Thiamine Hydrocholoride Pyridoxine Hydrochloride Riboflavin Niacin p-Aminobenzoic Acid Biotin Calcium Pantothenate Dipotassium phosphate Monopotassium phosphate Mangesium sulphate Sodium Chloride Ferrous Sulphate Manganese Sulphate |
12.00 40.00 20.00 0.20 0.20 0.02 0.02 0.02 0.002 1.004 0.002 0.002 0.0002 0.0000008 0.0004 1.00 1.00 0.40 0.02 0.02 0.02 |
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Final pH (at 25°C) : 6.8 + 0.2. |
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Directions |
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Suspend 7.5 gms. in 100 ml. distilled water. Boil to dissolve the medium completely. Mix well to distribute the slight precipitate evenly. For assay, dispense 5 ml medium per assay tube (containing increasing amounts of standard or the unknown) and make total volume to 10 ml with distilled water. Sterilize by autoclaving at 121°C for 10 minutes. Cool immediately |
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