BF 037 

VITAMINO GROWTH SUPPLEMENT

Formula

Ingredients 

(per vial sufficient for 500 ml. of medium)

Part I 

Vitamin B12 

L-glutamine 

Adenine sulphate 

Guanine hydrochloride 

p-amino benzoic acid (PABA) 

L-cystine 

NAD (Coenzyme I) 

Cocarboxylase 

Ferric nitrate 

Thiamine hydrochloride 

Cysteine hydrochloride 

Dextrose 

Part II (Rehydrating fluid) 

Dextrose 

Distilled water 

 

0.10 mg

100.00 mg

10.00 mg

0.30 mg

0.13 mg

11.00 mg

25.00 mg

1.00 mg

0.20 mg

0.03 mg

259.00 mg

1.00 gm

 

1.00 gm

10.00 ml

Directions

Dissolve the contents of Part I in 10 ml of Part II Rehydrating fluid. Aseptically add this to 240 ml of sterile, molten G.C. Agar Base (B 1049) along with 250 ml of sterile 2% haemoglobin solution / Transgrow Medium Base (B078) / Mycoplasma Urogenital Broth Base (B1233). Mix gently and pour into sterile Petri plates.

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