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B997 |
COLUMBIA C.N.A. AGAR |
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Formula |
B997 |
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Ingredients |
gms/lit. |
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Biopeptone Tryptic digest of beef heart Corn starch Sodium chloride Colistin sulphate Nalidixic acid Agar |
20.00 3.00 1.00 5.00 0.01 0.015 10.00 |
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Final pH (at 25°C) : 7.3 + 0.2 |
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Directions |
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Suspend 39 gms. of B997 in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool to 50°C and aspetically add 5% v/v sterile, defibrinated blood. Mix well and pour into sterile petri plates. |
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