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B993 |
COLONISATION MEDIUM W/DULBECCO’S PHOSPHATE BUFFER |
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Formula |
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Ingredients |
gms/lit. |
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Pancreatic digest of gelatin Peptic digest of animal tissue Brain heart infusion,solids Mannose Glucose Sodium chloride Disodium hydrogen phosphate Bile salts mixture Potassium chloride Potassium dihydrogen orthophosphate Calcium chloride Manganese chloride |
0.20 0.08 0.08 1.00 0.04 5.67 0.17 0.10 0.14 0.14 0.07 0.07 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 7.76 gms. in 1000 ml. distilled water. Mix thoroughly. Sterilize by filtration through a 0.45 um membrane. Dispense aseptically in sterile tubes or flasks. |
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