B932 

ASHBY’S MANNITOL AGAR

Formula 

B932 

Ingredients 

gms/lit.

Mannitol

Dipotassium phosphate 

Magnesium sulphate 

Sodium chloride

Potassium sulphate 

Calcium carbonate  

Agar 

20.00 

0.20 

0.20 

0.20

0.10

5.00

15.00 

Final pH (at 25°C) : 7.4 + 0.2

Directions

Suspend 40.7 gms. in 1000 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121° C) for 15 minutes.

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