B903

A.A.T.C.C. MINERAL SALTS AGAR

Formula

Ingredients

gm/lit. 

Ammonium nitrate 

Monopotassium phosphate 

Dipotassium phosphate 

Magnesium sulphate 

Ferrous sulphate 

Agar

3.00

2.50

2.00

0.20

0.10

20.00

Final pH (at 25°C) : 5.6 + 0.2

Directions 

Suspend 27.8 gms. in 1000 ml. distilled water. Add 7.5 gms. of dextrose if the medium is to be used for test ing with Aspergillus niger. Boil to dissolve the medium completely. Dispense in 7 ml. amounts in test tubes or 40 ml. amounts in bottles or flasks. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes.

Print This Page

Close Window