B903 |
A.A.T.C.C. MINERAL SALTS AGAR |
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Formula |
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Ingredients |
gm/lit. |
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Ammonium nitrate Monopotassium phosphate Dipotassium phosphate Magnesium sulphate Ferrous sulphate Agar |
3.00 2.50 2.00 0.20 0.10 20.00 |
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Final pH (at 25°C) : 5.6 + 0.2 |
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Directions |
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Suspend 27.8 gms. in 1000 ml. distilled water. Add 7.5 gms. of dextrose if the medium is to be used for test ing with Aspergillus niger. Boil to dissolve the medium completely. Dispense in 7 ml. amounts in test tubes or 40 ml. amounts in bottles or flasks. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. |
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