B898 

LEVINTHAL’s MEDIUM BASE

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Beef extract 

Sodium chloride 

Agar 

10.00

10.00

5.00

20.00

Final pH (at 25°C) : 7.6 + 0.2

Directions 

Suspend 45 gms. in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Dispense in 100 ml amounts and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and add 5 ml sterile rabbit for human blood to 100 ml medium. Heat the mixture in boiling water bath. Allow the deposits to sterile and dispense clear supernatent.

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