B898 |
LEVINTHAL’s MEDIUM BASE |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Beef extract Sodium chloride Agar |
10.00 10.00 5.00 20.00 |
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Final pH (at 25°C) : 7.6 + 0.2 |
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Directions |
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Suspend 45 gms. in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Dispense in 100 ml amounts and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and add 5 ml sterile rabbit for human blood to 100 ml medium. Heat the mixture in boiling water bath. Allow the deposits to sterile and dispense clear supernatent. |
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