B895 |
LIQUOID BROTH |
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Formula |
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Ingredients |
gms/lit. |
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Calf brain, infusion from Beef heart,infusion from Proteose peptone Sodium chloride Disodium phosphate Dextrose Sodium polyanethol sulphonate |
200.00 250.00 10.00 5.00 2.50 2.00 0.50 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 37.5 gms. in 1000ml. distilled water. Heat if necessary to ensure complete solution. Dispense in two bottles or tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If desired 1g/lit. agar can be added to encourage growth to anaerobic organisms. For best results, use the medium on the day it is prepared otherwise boil or steam it to remove dissolved oxygen just before use. |
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