B895 

LIQUOID BROTH

Formula

Ingredients 

gms/lit.

Calf brain, infusion from

Beef heart,infusion from 

Proteose peptone 

Sodium chloride 

Disodium phosphate 

Dextrose 

Sodium polyanethol sulphonate 

200.00

250.00

10.00

5.00

2.50

2.00

0.50

Final pH (at 25°C) : 7.4 + 0.2

Directions 

Suspend 37.5 gms. in 1000ml. distilled water. Heat if necessary to ensure complete solution. Dispense in two bottles or tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If desired 1g/lit. agar can be added to encourage growth to anaerobic organisms. For best results, use the medium on the day it is prepared otherwise boil or steam it to remove dissolved oxygen just before use.

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