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B893 |
LISTERIA ENRICHMENT BROTH, MODIFIED |
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Formula |
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Ingredients |
gms/lit. |
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Tryptose Yeast extract Beef extract Sodium chloride Disodium hydrogen phosphate Monopotassium hydrogen phosphate Esculin Nalidixic acid Acriflavin hydrochloride (Trypaflavin) |
10.00 5.00 5.00 20.00 9.60 1.35 1.00 0.02 0.012 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 52 gms. in 1000ml. distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes |
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