B892 

LISTERIA ENRICHMENT MEDIUM (UVM)

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate 

Proteose peptone 

Beef extract 

Yeast extract 

Sodium chloride 

Monopotassium dihydrogen phosphate 

Disodium hydrogen phosphate 

Esculin 

Nalidixic acid 

5.00

5.00

5.00

5.00

20.00

1.35

12.00

1.00

0.02

Final pH (at 25°C) : 7.4 + 0.2

Directions 

Suspend 54.37 gms. in 1000ml. distilled water.Heat to boiling to dissolve the medium completely.Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add filter sterilized solution of acriflavin hydrochloride to a final concentration of 12 mg per litre of medium or 25 mg per litre as per the requirement.

Print This Page

Close Window