B892 |
LISTERIA ENRICHMENT MEDIUM (UVM) |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Proteose peptone Beef extract Yeast extract Sodium chloride Monopotassium dihydrogen phosphate Disodium hydrogen phosphate Esculin Nalidixic acid |
5.00 5.00 5.00 5.00 20.00 1.35 12.00 1.00 0.02 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 54.37 gms. in 1000ml. distilled water.Heat to boiling to dissolve the medium completely.Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add filter sterilized solution of acriflavin hydrochloride to a final concentration of 12 mg per litre of medium or 25 mg per litre as per the requirement. |
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