B886 

LOEFFLER SERUM MEDIUM BASE

Formula

Ingredients 

gms/lit.

Heat muscle, infusion from 

Peptic digest of animal tissue 

Sodium chloride 

Dextrose 

Egg powder 

0.72

0.71

0.36

0.71

7.50

Final pH (at 25°C) : 7.6 + 0.2

Directions 

Suspend 10 gms. in 250ml. distilled water at a temperature of 45°C. Mix well and dispense into tubes. Coagulate and sterilize by inspissation for 15 minutes at 80 to 90°C for steaming at 100°C for 10-15 minutes.

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