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B876 |
M-ENDO AGAR LES |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Peptic digest of animal tissue Tryptose Yeast extract Lactose Dipotassium phosphate Monopotassium phosphate Sodium chloride Sodium deoxycholate Sodium lauryl sulphate Sodium sulphite Basic fuchsin Agar |
3.70 3.70 7.50 1.20 9.40 3.30 1.00 3.70 0.10 0.05 1.60 0.80 15.00 |
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Final pH (at 25°C) : 7.2 + 0.2 |
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Directions |
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Suspend 51 gms. in 980 ml. distilled water. Boil to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45°C and aseptically add 20 ml. of 95% ethanol. Mix and dispense 4 ml. amounts into 60 mm petri plates. In large plates, use sufficient medium to give an 1.5% mm depth. DO NOT EXPOSE PLATES TO DIRECT SUNLIGHT. |
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