B875 

M-ENDO BROTH

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue 

Yeast extract 

Lactose 

Dipotassium phosphate 

Basic fuchsin 

Sodium sulphite 

20.00

6.00

25.00

7.00

1.00

2.50

Final pH (at 25°C) : 7.5 + 0.2

Directions 

Suspend 61.5 gms. in 1000ml. distilled water. Heat to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 5 minutes. Cool and use as required in membrane filtration technique. The medium should be used on the same day of its rehydration.

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