B872 

M-ENTEROCOCCUS AGAR BASE, MODIFIED

Formula

Ingredients 

gms/lit.

Pancreatic digest of gelatin

Yeast extract

Sodium chloride

Esculin

Sodium azide

Cycloheximide

Nalidixic acid

Agar

10.00

30.00

15.00

1.00

0.15

0.05

0.25

15.00

Final pH (at 25°C) : 7.1 + 0.2

Directions 

Suspend 71.4 gms. in 1000ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool to 45°C and aseptically add 15ml of sterile 1% TTC Solution. Mix well and pour into sterile petri plates.

Print This Page

Close Window