B872 |
M-ENTEROCOCCUS AGAR BASE, MODIFIED |
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Formula |
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Ingredients |
gms/lit. |
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Pancreatic digest of gelatin Yeast extract Sodium chloride Esculin Sodium azide Cycloheximide Nalidixic acid Agar |
10.00 30.00 15.00 1.00 0.15 0.05 0.25 15.00 |
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Final pH (at 25°C) : 7.1 + 0.2 |
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Directions |
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Suspend 71.4 gms. in 1000ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool to 45°C and aseptically add 15ml of sterile 1% TTC Solution. Mix well and pour into sterile petri plates. |
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