B857 |
M17 AGAR BASE/WITHOUT LACTOSE |
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Formula |
B857 |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Peptic digest of animal tissue Papaic digest of soyabean meal Biopeptone Yeast extract Meat extract Beef extract Ascorbic acid Magnesium sulphate Lactose Disodium – β-glycerophosphate Agar |
5.00 - 5.00 - 2.50 5.00 - 0.50 0.25 - 19.00 11.00 |
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Final pH (at 25°C) : |
6.9 + 0.2 |
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Directions : |
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Suspend 48.25 gms. of B857 in 950ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45°C and aseptically add 50ml of 10% w/v separately sterilized lactose solution to B857. Mix well and dispense as desired |
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