B857

M17 AGAR BASE/WITHOUT LACTOSE  

Formula 

B857 

Ingredients 

gms/lit. 

Casein enzymic hydrolysate

Peptic digest of animal tissue

Papaic digest of soyabean meal

Biopeptone

Yeast extract

Meat extract

Beef extract

Ascorbic acid

Magnesium sulphate

Lactose

Disodium – β-glycerophosphate

Agar

5.00

-

5.00

-

2.50

5.00

-

0.50

0.25

-

19.00

11.00

Final pH (at 25°C) : 

6.9 + 0.2 

Directions :

 Suspend 48.25 gms. of B857 in 950ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45°C and aseptically add 50ml of 10% w/v separately sterilized lactose solution to B857. Mix well and dispense as desired

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