B855

M17 AGAR BASE/ WITH GLYCEROPHOSPHATE.

Formula 

B855 

Ingredients 

gms/lit. 

Casein enzymic hydrolysate

Peptic digest of animal tissue

Papaic digest of soyabean meal

Biopeptone

Yeast extract

Meat extract

Beef extract

Ascorbic acid

Magnesium sulphate

Lactose

Disodium – β-glycerophosphate

Agar

-

5.00

5.00

2.50

-

5.00

0.50

0.25

5.00

19.00

10.00

Final pH (at 25°C) : 

7.1 + 0.2 

Directions :

Suspend 52.25 gms. of B855 in 1000 ml. distilled water.   Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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