B849 

AZOTOBACTER AGAR (MANNITOL) 

Formula 

Ingredients 

gms/lit.

Dipotassium phosphate

Magnesium sulphate 

Sodium chloride 

Ferrous sulphate 

Soil extract 

Mannitol

Agar 

1.00

0.20

0.20

Trace 

5.00

20.00

15.00 

Final pH (at 25°C) : 8.3 + 0.2 

Directions 

Suspend 41.4 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If slight precipitate occurs after autoclaving, distribute it evenly before pouring into sterile plates.

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