B849 |
AZOTOBACTER AGAR (MANNITOL) |
|
Formula |
||
Ingredients |
gms/lit. |
|
Dipotassium phosphate Magnesium sulphate Sodium chloride Ferrous sulphate Soil extract Mannitol Agar |
1.00 0.20 0.20 Trace 5.00 20.00 15.00 |
|
Final pH (at 25°C) : 8.3 + 0.2 |
||
Directions |
||
Suspend 41.4 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If slight precipitate occurs after autoclaving, distribute it evenly before pouring into sterile plates. |
||