B846 

ASPERGILLUS DIFFERENTIATION MEDIUM BASE 

Formula 

Ingredients 

gms/lit. 

Peptic digest of animal tissue

Yeast extract 

Ferric ammonium citrate 

Dichloran 

Agar 

10.00

20.00

0.50

0.002

15.00 

Final pH (at 25°C) : 6.3 + 0.2 

Directions 

Suspend 22.75 gms. in 500 ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool around 50°C and aseptically add sterile rehydrated contents of 1 vial of Chloramphenicol Selective Supplement. Mix well. 

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