B840 

ARGININE DIHYDROLASE BROTH 

Formula 

Ingredients 

gms/lit. 

Peptic digest of animal tissue

Sodium chloride 

Dipotassium hydrogen phosphate 

L-arginine 

Bromo cresol purple 

Agar 

1.00

5.00

0.30

10.00

0.016

3.00 

Final pH (at 25°C) : 7.2 + 0.2 

Directions 

Suspend 19.3 gms. in 1000 ml. distilled water. Heat to dissolve the medium completely and distribute 13 X 100 mm tubes. Sterilize by autoclaving at 10 lbs pressure (115°C) for 15 minutes. Allow the tubes to cool in an upright position.

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