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B835 |
ANDRADE PEPTONE WATER WITH MEAT EXTRACT |
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Formula |
B835 |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Meat extract Sodium chloride Andrade indicator |
10.00 3.00 5.00 0.10 |
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Final pH (at 25°C) : |
7.1 + 0.2 | ||
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Directions |
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Suspend 18.1 gms. of B835 in 1000 ml. distilled water. Dissolve the medium and dispense in test tubes containing inverted Durham’s tubes.Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to room temperature and aseptically add sterile stock solution of carbohydrate to a final concentration of 5% to 1.0% (w/v) |
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