B830 

MUG MACCONKEY AGAR

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Lactose

Bile salt mixture

Sodium chloride

Neutral red

Crystal violet

4-Methylumbelliferyl β-D- glucuronide (MUG)

Agar

20.00

10.00

1.50

5.00

0.03

0.001

0.10

15.00

Final pH (at 25°C) : 7.1 + 0.2

Directions 

Suspend 51.63 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize By autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well before pouring.

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