B828

MRS AGAR MODIFIED (LACTOBACILLI HETEROFERM SCREEN AGAR )

Formula 

B828 

Ingredients 

gms/lit. 

Dextrose 

Proteose peptone 

Yeast extract 

Sodium acetate 

2-Phenylethyl alcohol 

Ammonium citrate 

Dipostassium phosphate 

Magnessium sulphate 

Manganese sulphate 

Bromo cresol green

Cycloheximide 

Agar

20.00 

10.00 

5.00 

5.00 

3.00 

2.00 

2.00 

0.10 

0.05 

 0.04 

0.004 

 15.00 

Final pH (at 25°C) : 

5.5 + 0.2 

Directions 

Suspend 62.2 grams in 1000 ml distilled water containing 1 ml Polysorbate 80. Mix thoroughly. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If necessary adjust the pH with glacial acetic acid after sterilization.

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