B828 |
MRS AGAR MODIFIED (LACTOBACILLI HETEROFERM SCREEN AGAR ) |
||
Formula |
B828 |
||
Ingredients |
gms/lit. |
||
Dextrose Proteose peptone Yeast extract Sodium acetate 2-Phenylethyl alcohol Ammonium citrate Dipostassium phosphate Magnessium sulphate Manganese sulphate Bromo cresol green Cycloheximide Agar |
20.00 10.00 5.00 5.00 3.00 2.00 2.00 0.10 0.05 0.04 0.004 15.00 |
||
Final pH (at 25°C) : |
5.5 + 0.2 |
||
Directions |
|||
Suspend 62.2 grams in 1000 ml distilled water containing 1 ml Polysorbate 80. Mix thoroughly. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If necessary adjust the pH with glacial acetic acid after sterilization. |
|||