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B822 |
YEAST EXTRACT ROSE BENGAL BROTH BASE |
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Formula |
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Ingredients |
gms/lit. |
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Yeast extract Disodium phosphate Bile salt Sodium chloride Magnesium sulphate Sodium pyruvate Rose bengal |
5.00 17.25 2.00 1.00 0.01 1.00 0.04 |
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Final pH (at 25°C) : 7.9 + 0.2 |
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Directions |
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Suspend 26.3 gms. in 900ml. distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 20-25 C and aseptically add 100 ml. of 4% filter sterilized sorbose solution. Mix well and dispense aseptically as desired. |
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