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B794 |
TRYPTONE PEPTONE GLUCOSE EXTRACT BROTH BASE (w/o/Trypsin) |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Yeast extract Peptic digest of animal tissue Dextrose Sodium thioglycollate |
50.00 20.00 5.00 4.00 1.00 |
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Final pH (at 25°C) : 7.2 + 0.2 |
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Directions |
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Suspend 80 gms. in 1000ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Refrigerate the medium until use. Before inoculation add 1.5% filter sterilized Trypsin solution to a final concentration of 0.1%. |
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