B786

TRIPLE SUGAR IRON AGAR

Formula 

B786 

Ingredients 

gms/lit. 

Pepetones (casein & beef)

Yeast extract

Beef extract 

Lactose monohydrate 

Sucrose 

D-Glucose monohydrate 

Sodium chloride 

Ammonium iron(III) citrate 

Phenol red 

Agar 

20.00 

 3.00 

3.00 

10.00 

10.00 

1.00 

5.00 

0.30

0.025 

12.00 

Final pH (at 25°C) : 7.4 + 0.2

Directions 

Suspend 65 gms. in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Mix well and distribute into test tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow the medium to set in sloped form with a butt about 1 inch long .

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