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B780 |
THIOGEL MEDIUM |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Papaic digest of soyabean meal Dextrose Sodium chloride Sodium thioglycollate L-cystine Sodium sulphite Gelatin Agar |
17.00 3.00 6.00 2.50 0.50 0.25 0.10 50.00 0.70 |
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Final pH (at 25°C) : 7.0 + 0.2 |
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Directions |
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Suspend 80 gms. in 1000ml. distilled water, preheated to a temperature of 50°C. Mix well and allow to stand for 5 minutes. Heat to boiling to dissolve the medium completely. Dispense in test tubes filling them upto half of the tubes. Sterilize by autoclaving at 12 lbs pressure (118°C) for 15 minutes. |
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