B777 

THIOGLYCOLLATE Broth w/LIVER EXTRACT(B.Q.VACCINE MEDIUM)

Formula

Ingredients 

gms/lit.

Peptice Digest of Animal Tissue 

Liver Tissue 

Muscle Tissue 

Sodium chloride 

Di-Potassium phosphate 

Sodium thioglycollate 

10.00

250.00

250.00

5.00

4.00

1.00

Final pH (at 25°C) : 8.2 + 0.2

Directions 

Suspend 30.00 gms. in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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