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B758 |
SULPHATE BROTH w/o NaCl |
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Formula |
B758 |
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Ingredients |
gms/lit. |
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Yeast extract Ascorbic acid Magnesium sulphate Dipotassium phosphate Ferrous ammonium sulphate |
1.00 0.10 0.20 0.01 0.10 |
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Final pH (at 25°C) : |
7.4 + 0.2 |
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Directions |
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Suspend 1.4 gms. of B758 in 1000ml. distilled water. Add 4 ml. of sodium lactate. Heat to dissolve the medium completely. Dispense, preferably in screw –capped tubes in 9 ml. amounts. Sterilize by autoclaving at 15 lbs pressure (121°C) for 10 minutes. |
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