B750 

STREPTOCOCCUS LACTIS DIFFERENTIAL AGAR BASE

Formula

Ingredients 

gms/lit.

Nonfat (skim) milk

Peptonized milk

Dextrose

Agar

10.00

2.50

5.00

15.00

Final pH (at 25°C) : 6.6 + 0.2

Directions 

Suspend 32.5 gms. in 1000ml. distilled water. Heat to boiling with stirring to dissolve the medium completely. Sterilize by autoclaving at 10 lbs pressure (115°C) for 12 minutes. Cool to 45°C and aseptically add (30 minutes steam sterilized solutions) 10 ml. of 10% potassium fericyanide and 10 ml. of citrate solution containing 0.25g ferric citrate and 0.25g sodium citrate. Gently mix and pour into the sterile petri plates. Dry the plates in dark for 24 hours at 30°C.

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