B742 

MEHLMAN’S MAINTENANCE MEDIUM

Formula

Ingredients 

gms/lit.

Proteose peptone

Yeast extract

Casein enzymic hydrolysate

Dipotassium phosphate

Starch, soluble

Ammonium sulphate

Neutral red

Agar

15.00

7.50

5.00

5.00

1.00

1.50

0.02

3.00

Final pH (at 25°C) : 7.3 + 0.2

Directions 

Suspend 38 gms. in 1000ml distilled water. Heat to boiling to dissolve the medium completely. Dispense in 8ml amounts in screw capped tubes. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool to 45- 50°C and aseptically add 0.2 ml filter sterilized 2.5 % sodium sulphite solution to each tube. Mix gently.

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