B742 |
MEHLMAN’S MAINTENANCE MEDIUM |
|
Formula |
||
Ingredients |
gms/lit. |
|
Proteose peptone Yeast extract Casein enzymic hydrolysate Dipotassium phosphate Starch, soluble Ammonium sulphate Neutral red Agar |
15.00 7.50 5.00 5.00 1.00 1.50 0.02 3.00 |
|
Final pH (at 25°C) : 7.3 + 0.2 |
||
Directions |
||
Suspend 38 gms. in 1000ml distilled water. Heat to boiling to dissolve the medium completely. Dispense in 8ml amounts in screw capped tubes. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool to 45- 50°C and aseptically add 0.2 ml filter sterilized 2.5 % sodium sulphite solution to each tube. Mix gently. |
||