B738 

STANDARD NUTRIENT AGAR

Formula

Ingredients 

gms/lit.

Peptic deigest of lean meat

Beef extract 

Sodium chloride 

Agar 

500.00

10.00

5.00

20.00

Final pH (at 25°C) : 7.6 + 0.2

Directions 

Suspend 45 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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