B736 

STANDARD METHODS AGAR with TWEEN 80 & LECITHIN

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate

Yeast extract

Dextrose

Lecithin

Polysorbate 80 (Tween 80)

Agar

5.00

2.50

1.00

0.70

5.00

15.00

Final pH (at 25°C) : 7.0 + 0.2

Directions 

Suspend 29.2 gms. in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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