B735 

MIDDLEBROOK 7H9 AGAR BASE A

Formula

Ingredients 

gms/lit.

Amonium sulphate

Disodium phosphate

Monopotassium Phosphate

Sodium citrate

Magnesium sulphate

Calcium chloride

Zinc sulphate

Copper sulphate

Ferric ammonium citrate

Sodium glutamate

Pyridoxine

Biotin

Malacite Green

Agar

0.50

2.50

1.00

0.10

0.05

0.0005

0.001

0.001

0.04

0.50

0.001

0.0005

0.001

15.00

Final pH (at 25°C) : 6.6 + 0.2

Directions 

Suspend 9.85 gms. in 450 ml distilled water. Add 1ml. glycerol if desired. Heat to boil to to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 10 minutes. Cool to 50-55°C or below and aseptically add 50 ml. of OADC Enrichment Supplement. Mix well before dispensing.

Print This Page

Close Window