B735 |
MIDDLEBROOK 7H9 AGAR BASE A |
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Formula |
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Ingredients |
gms/lit. |
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Amonium sulphate Disodium phosphate Monopotassium Phosphate Sodium citrate Magnesium sulphate Calcium chloride Zinc sulphate Copper sulphate Ferric ammonium citrate Sodium glutamate Pyridoxine Biotin Malacite Green Agar |
0.50 2.50 1.00 0.10 0.05 0.0005 0.001 0.001 0.04 0.50 0.001 0.0005 0.001 15.00 |
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Final pH (at 25°C) : 6.6 + 0.2 |
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Directions |
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Suspend 9.85 gms. in 450 ml distilled water. Add 1ml. glycerol if desired. Heat to boil to to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 10 minutes. Cool to 50-55°C or below and aseptically add 50 ml. of OADC Enrichment Supplement. Mix well before dispensing. |
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