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B729 |
MIDDLEBROOK 7H10 AGAR BASE |
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Formula |
B729 |
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Ingredients |
gms/lit. |
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Amonium sulphate L-Glutamic acid Monopotassium Phosphate Disodium phosphate Sodium citrate Ferric ammonium citrate Magnesium sulphate Calcium chloride Zinc sulphate Copper sulphate Pyridoxine hydrochloride Biotin Malachite green Agar |
0.50 0.50 1.50 1.50 0.40 0.04 0.025 0.0005 0.001 0.001 0.001 0.0005 0.00025 15.00 |
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Final pH (at 25°C) : 6.6 + 0.2 |
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Directions |
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Suspend 19.47 gms. of B729 in 900 ml distilled water containing 5ml. glycerol. Boil to dissolve the medium completely. Distribute in 180ml. amounts in flasks and sterilize at 15 lbs pressure (121°C) for 10 minutes. To each cooled sterile 180ml. medium aseptically add 20ml. Middlebrook OADC Growth Supplement. Mix well and pour into sterile screw capped tubes or containers. NOTE : Keep prepared medium in the dark before and after inoculation. |
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