B728 

SPORULATION BROTH 

(ARRET AND KRISHBAUM MEDIUM–A.K. AGAR NO. 2)

Formula

Ingredients 

gms/lit.

Pancreatic digest of gelatin

Casein enzymic hydrolysate

Yeast extract

Beef extract

Dextrose

Manganous sulphate

6.00

4.00

3.00

1.50

1.00

0.30

Final pH (at 25°C) : 6.6 + 0.2

Directions 

Suspend 15.8 gms. in 1000ml. distilled water. Boil with stirring to dissolve the medium completely. Dispense in 300ml. amounts in Roux or other suitable bottles. Sterilize by autoclaving at 15 lbs pressure (121° C) for 15 minutes. 

NOTE : Do not autoclave till the medium has been completely dissolved.

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