B728 |
SPORULATION BROTH (ARRET AND KRISHBAUM MEDIUM–A.K. AGAR NO. 2) |
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Formula |
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Ingredients |
gms/lit. |
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Pancreatic digest of gelatin Casein enzymic hydrolysate Yeast extract Beef extract Dextrose Manganous sulphate |
6.00 4.00 3.00 1.50 1.00 0.30 |
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Final pH (at 25°C) : 6.6 + 0.2 |
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Directions |
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Suspend 15.8 gms. in 1000ml. distilled water. Boil with stirring to dissolve the medium completely. Dispense in 300ml. amounts in Roux or other suitable bottles. Sterilize by autoclaving at 15 lbs pressure (121° C) for 15 minutes. NOTE : Do not autoclave till the medium has been completely dissolved. |
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