B725 

SODIUM AZIDE CRYSTAL VIOLET BLOOD AGAR BASE

Formula

Ingredients 

gms/lit.

Beef heart,infusion from

Casein enzymic hydrolysate

Sodium chloride

Glucose

Sodium azide

Crystal violet

Agar

500.00

20.00

5.00

0.20

0.30

0.002

15.00

Final pH (at 25°C) : 7.0 + 0.2

Directions 

Suspend 50.5 gms. in 950ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 5% v/v sterile defibrinated blood. Mix well and pour into sterile petri plates.

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