B725 |
SODIUM AZIDE CRYSTAL VIOLET BLOOD AGAR BASE |
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Formula |
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Ingredients |
gms/lit. |
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Beef heart,infusion from Casein enzymic hydrolysate Sodium chloride Glucose Sodium azide Crystal violet Agar |
500.00 20.00 5.00 0.20 0.30 0.002 15.00 |
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Final pH (at 25°C) : 7.0 + 0.2 |
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Directions |
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Suspend 50.5 gms. in 950ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 5% v/v sterile defibrinated blood. Mix well and pour into sterile petri plates. |
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