B721 

SENSITIVITY TEST MEDIUM

Formula

Ingredients 

gm/lit.

Peoteose peptone

Veal,infusion from 

Dextrose 

Sodium chloride 

Disodium phosphate 

Sodium acetate 

Adenine sulphate 

Guanine 

Uracil 

Xanthine 

Agar 

10.00

10.00

10.00

3.00

2.00

1.00

0.01

0.01

0.01

0.01

15.00

Final pH (at 25°C) : 7.3 + 0.2

Directions 

Suspend 51 gm. in 1000ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 12-15 lbs pressure (118-121°C) for 15 minutes. Cool to 50°C and add sterile serum or blood aseptically if desired. Mix well and pour into sterile petri plates.

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