B706 

MILK AGAR WITH CETRIMIDE (TWIN PACK)

Formula

Ingredients 

gms/lit.

Part A :

Skim milk Powder 

Part B :

Peptic digest of animal tissue Sodium

chloride 

Yeast extract 

Cetrimide 

Agar 

 

133.33

 

3.33

1.67

1.00

0.40

20.00

Final pH (at 25°C) : 7.3 + 0.2

Directions 

Suspend 26.4 gms. of Part B in 250 ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 20 minutes. Suspend 133 gms. of Part A in 750 ml of distilled water and sterilize by autoclaving at 15 lbs pressure (121°C) for 5 minutes. After autoclaving mix Part A and B and pour into sterile petri plates.

Print This Page

Close Window