B703

ROSE BENGAL CHLORAMPHENICOL AGAR

Formula 

B703 

Ingredients 

gms/lit.

Mycological peptone

Dextrose

Monopotassium phosphate

Magnesium sulphate 

Rose horou 

Chloramphenicol

Agar 

5.00

10.00

1.00

0.50

 0.05

0.10

15.50

Final pH (at 25°C) : 7.2 + 0.2

Directions

Suspend 32.15 gms. of B703 in 1000ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45°C & add 2 ml of rehydrated Chloramphenicol selective supplement for each 500 ml of B307. Mix thoroughly & pour into sterile petri plate.

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