B690 

MODIFIED BRUCELLA AGAR BASE

Formula

Ingredients 

gms/lit

Casein enzymic hydrolysate

Peptic digest of animal tissue

Sodium chloride

Glucose

Agar

10.00

5.00

5.00

10.00

15.00

Final pH (at 25°C) : 7.5 + 0.2

Directions 

Suspend 45gms. in 890ml distilled water when Campylobacter Supplement I (Blaser Wang) is to be used or in 940ml distilled water when Campylobacter Supplement II (Butzler) is to be used. Boil to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool to 45-50°C.  Mix well before pouring into sterile petri plates.

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