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B672 |
MYP AGAR BASE |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Meat extract D-Mannitol Sodium chloride Phenol red Agar |
10.00 1.00 10.00 10.00 0.025 15.00 |
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Final pH (at 25°C) : 7.1 + 0.2 |
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Directions |
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Suspend 46 gms. in 900 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 55°C. Aseptically add sterile Polymyxin B Sulphate solution to a final concentration of 100 units per ml. & 100 ml. sterile Egg Yolk Emulsion per 1000 ml. medium. Mix well & pour into sterile petri plates. |
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