B669 

M-BISMUTH SULPHITE BROTH

Formula

Ingredients 

gms/lit

Peptic digest of animal tissue

Beef extract

Dextrose

Disodium phosphate

Ferrous sulphate

Bismuth sulphite indicator

Brilliant green

20.00

10.00

10.00

8.00

0.60

16.00

0.05

Final pH (at 25°C) : 7.7 + 0.2

Directions 

Suspend 64.65 gms. in 1000ml distilled water. Boil to dissolve the medium completely. Excessive heating destroys the selective properties of the medium. DO NOT AUTOCLAVE. The medium usually contains flocculent precipitate which should be dispersed evenly by swirling the flask just before use. Use 2ml of the medium per pad.

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