B666 

PEPTONE IRON AGAR

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Proteose peptone

Ferric ammonium citrate

Sodium glycerophosphate

Sodium thiosulphate

Agar

15.00

5.00

0.50

1.00

0.08

15.00

Final pH (at 25°C) : 6.7 + 0.2

Directions 

Suspend 36.58 gms. in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Dispense in test tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow the tubed medium to cool in an upright position.

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