B664 

PANTOTHENATE INOCULUM BROTH

Formula

Ingredients 

gms/lit.

Peptonized milk

Yeast extract 

Dextrose 

Monopotassium phosphate 

Tomato juice (100 ml) 

Polysorbate 80 

15.00

5.00

10.00

2.00

5.00

1.00

Final pH (at 25°C) : 6.8 + 0.2

Directions 

Suspend 38 gms. in 1000ml. distilled water. Boil to dissolve the medium completely. Distribute in tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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